IMMUNOLOGICAL SPECIFICITY OF HUMAN INSULIN: APPLICATION TO IMMUNOASSAY OF INSULIN

Immunological specificity of human insulin: application to immunoassay of insulin.

Previous investigations have revealed evidence for species-specificity among various mammalian insulins in immunologic reactions (1-5). For this reason, immunoassay of plasma insulin (610) in any species generally requires that the insulin employed in standard solutions be derived from the same species. Immunoassay of plasma insulin in man has been carried out by comparing the effects of unknow...

Immunoassay of insulin with insulin antibody preciptate.

Micromethods for the assay of insulin based on the reaction between 131I-labelled insulin and insulin antibody have been described by Yalow & Berson (1960) and Grodsky & Forsham (1960). In principle they depend on the use of insulin antibody to obtain samples of insulin from mixtures prepared by adding standard or unknown solutions of unlabelled insulin to a fixed amount of 131I-labelled insuli...

Immunological specificity of Staphylococal penicillinase

Optimization and Construction of Human Insulin-like Growth Factor 1 Gene related to human health

Background and aims: Laron syndrome is a disease that treated by Insulin-like Growth Factor 1 (IGF-1). This protein is a single chain and has three disulfide bonds. People with Laron syndrome have low rates of cancer and diabetes, although they appear to be at increased risk of casual death due to their stature. IGF-1 is synthesized by many tissues and is secreted from liver as an endocrine hor...

In-vitro Differentiation of Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells to Insulin-Producing Cells

  Background & Objective: Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability to differentiate into Insulin-Producing Cells (IPCs). In this study, Human Umbilical Mesenchymal Stem Cells (HUMSCS) were in...